Adaptation to form bacteriochlorophyll in Rhodopseudomonas spheroides: changes in activity of enzymes concerned in pyrrole synthesis.

نویسنده

  • J LASCELLES
چکیده

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Mutant strains of Rhodopseudomonas spheroides lacking delta-aminolevulinate synthase: growth, heme, and bacteriochlorophyll synthesis.

Two mutant strains of Rhodopseudomonas spheroides were described which lacked delta-aminolevulinate synthase activity. They required delta-aminolevulinate for growth; they did not respond to protoporphyrin or magnesium photoporphyrin, and only poorly to hemin. Synthesis of cytochromes and heme by mutant H-4 was dependent upon delta-aminolevulinate; this strain did not form bacteriochlorophyll e...

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Photosynthetic membrane development in Rhodopseudomonas spheroides: incorporation of bacteriochlorophyll and development of energy transfer and photochemical activity.

I. Bleached cells of Rhodopseudomonas spheroides, obtained by aerobic growth in the light, rapidly synthesize bacteriochlororphyll and earotenoids when transferred to 4 % oxygen in the dark. Fluorescence excitation spectra of whole cells during the initial stages of pigment synthesis indicate that the major fraction of the newly synthesized bacterioehlorophyll is immediately incorporated into p...

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Coupling between bacteriochlorophyll and membrane protein synthesis in Rhodopseudomonas spheroides.

Bacteriochlorophyll-containing membranes from Rhodopseudomonas spheroides contain proteins with estimated molecular weights of 26,000, 22,000, 19,000, and 10,000-6,000 (proteins 9, 10, 11, and 15) when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins 9, 10, and 11 may be associated with the reaction center form of bacteriochlorophyll and protein 15, with the light...

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Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides.

Rhodopseudomonas spheroides can grow in a defined medium with either light or oxygen as an energy source. Cells grown anaerobically or at very low oxygen tensions are rich in the photosynthetic pigment bacteriochlorophyll, whereas this pigment is virtually absent in cells grown under high oxygen tensions. Aminolaevulinate synthetase, the first enzyme on the pathway to bacteriochlorophyll, appea...

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Bacteriochlorophyll Synthesis and the Ultrastructure of Wild Type and Mutant Strains of Rhodopseudomonas spheroides.

The ultrastructure of sectioned cells of mutant and wild type Rhodopseudomonas spheroides has been examined by electron microscopy. The characteristic vesicles associated with the presence of bacteriochlorophyll were found in wild type cells grown with low aeration. These were also found in mutant TA-R which forms bacteriochlorophyll under high aeration. None of the mutants with blocks in bacte...

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عنوان ژورنال:
  • The Biochemical journal

دوره 72  شماره 

صفحات  -

تاریخ انتشار 1959